written 6.7 years ago by
aishu.jp
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Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment".
I don't know about `offst()` function, what is the scale or direction, or whether you a…
Comment: Average biological replicates in RNA-seq
by
boczniak767
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@atpoint Should I first standardise data and then average replicates?
Comment: 3-factor DESeq2 trime-series analysis
by
gogeni5529
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0
Thans you @swbarnes2 for the answer.
I can see why trying to include all three parameters might be difficult to interpret. But we're inte…
Comment: 3-factor DESeq2 trime-series analysis
by
swbarnes2
★
1.4k
What does your PCA look like? A lot of times, I find that different cell lines are so different from each other, that they should not be d…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
yongjie.wang
•
0
Thanks for your reply.
" normFactors <- exp(-1 * offst(set2))
normFactors <- normFactors / exp(rowMeans(log(normFactors)))
normalizat…
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